Determination of Restriction Enzyme Activity when Cutting Fluorochrome Labeled DNA Molecules

Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA

Fluorescently labeled circular DNA molecules for DNA topology and topoisomerases

DNA topology plays essential roles in several fundamental biological processes, such as DNA replication, recombination, and transcription. Typically agarose gel electrophoresis is employed to study DNA topology. Since gel electrophoresis is time-consuming and labor intensive, it is desirable to develop other methods, such as fluorescence-based methods, for such studies. In this paper we report ...

Determination of Malassezia species isolated from patients with Pityriasis Versicolor using PCR-restriction enzyme method

Background and aim: Malassezia species are part of the resident skin flora of humans. These yeasts are associated with various superficial diseases, including seborrheic dermatitis, atopic dermatitis, dandruff, and psoriasis. Various DNA-based molecular methods have been recently described to differentiate species of Malassezia. In this survey, a simple, reliable, and cost effective PCR-b...

DNA cloning without restriction enzyme and ligase.

One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing "customized cohesive ends" by a combination of PCR primer design and lambda exonuclease digestion. These complementary cohesive ends can form h...

DNA-tension dependence of restriction enzyme activity reveals mechanochemical properties of the reaction pathway

Type II restriction endonucleases protect bacteria against phage infections by cleaving recognition sites on foreign double-stranded DNA (dsDNA) with extraordinary specificity. This capability arises primarily from large conformational changes in enzyme and/or DNA upon target sequence recognition. In order to elucidate the connection between the mechanics and the chemistry of DNA recognition an...